Comparative studies on oestrogen-induced rat uterus peroxidase and rat eosinophil peroxidase

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Comparative studies on oestrogen-induced rat uterus peroxidase and rat eosinophil peroxidase.

Rat eosinophil peroxidase and rat uterine peroxidase II showed similar electrophoretic mobilities, molecular weights, specific activities and spectral properties and could be purified by essentially identical techniques. Antibodies raised against the uterine enzyme strongly inhibited the eosinophil enzyme. It is suggested that rat eosinophil peroxidase and rat uterine peroxidase II may well be ...

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Spectral properties of the oestrogen-induced rat uterus peroxidase II and some of its derivatives.

1. The visible absorption spectrum of peroxidase II, isolated from the uterine tissue of oestradiol-treated rats, and some of its derivatives were recorded. The spectral properties of this enzyme are very similar to eosinophile peroxidase and lactoperoxidase, suggesting that these enzymes may have a similar form of haem as prosthetic group. 2. The uterine peroxidase is modified upon interaction...

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Eosinophil peroxidase-induced mast cell secretion

Eosinophil peroxidase (EPO) at relatively low levels (4-30 mU), when supplemented with H2O2 and a halide, induced mast cell degranulation. Histamine release occurred without concomitant release of the cytoplasmic marker lactic dehydrogenase (LDH), and this, together with ultrastructural studies, indicated a noncytotoxic effect comparable with that induced by other mast cell secretagogues. At pH...

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Peroxidase and cytochrome oxidase in rat tissues.

The presence of peroxidase in the uterus of the estrogeninjected ovariectomized rat and in the Walker carcinosarcoma No. 256 of rats has been reported previously (1, 2). In view of the fact that both these tissues undergo rapid proliferation, it was thought of interest to survey several other rat tissues to determine whether peroxidase might be generally associated with proliferation. Bancroft ...

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Glutathione peroxidase induced in rat retinas to counteract photic injury.

PURPOSE To examine the hypothesis that glutathione peroxidase (GPX) is induced at different time points after retinal exposure to light and localizes in different retinal cells. METHODS The rats were kept in cyclic light for 2 weeks before the experiments. The animals were maintained in 12-hour light-dark cycles, before and after exposure to intense white fluorescent light, for as long as 24 ...

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ژورنال

عنوان ژورنال: Biochemical Journal

سال: 1982

ISSN: 0264-6021

DOI: 10.1042/bj2070613